WebCD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4 + lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in ... WebELISA Capture: Biotin Mouse Anti-Human CD154 antibody (Cat. No. 552560) is useful as a detection antibody for a sandwich ELISA for measuring human CD154 (CD40L) protein levels.Biotin Mouse Anti-Human CD154 antibody should be titrated between 0.5 - 2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard …
CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE (12-1541 …
WebApr 15, 2013 · In brief, cells were indirectly magnetically labeled with CD154-biotin and antibiotin Microbeads and enriched by two sequential MS MACS columns (Miltenyi Biotec). If separation of Ag-specific T cells was combined with an intracellular staining, surface staining was performed on the first column, followed by fixation, permeabilization (Inside ... jpr south coors
Anti-CD154 Antibody Products Biocompare
WebThe CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads ... Webondary antibody. CD154 was stained with either anti– CD154-PE or anti-CD154-biotin and streptavidin-FITC (PharMingen). Either rat or hamster isotype controls were used and kept constant throughout the experiment. Unbound antibodies were removed from the sections by aspiration followed by 4 20-minute washes in PBA. Washed sections were then fixed WebMay 12, 2006 · The method relies on detection of CD154, a costimulatory cell surface protein that is expressed by CD4+ T cells upon activation, and can be used to purify live CD4+ T cells of diverse function ... jprs posh acme